Coding
Part:BBa_J51003:Design
Designed by: Peter Nguyen Group: iGEM06_Rice (2006-10-25)
Tsr MCP receptor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1504
Design Notes
The BB prefix and suffix sites are non-standard.
Primers used for isolation and construction (5'->3')
F: cggaattcgctctagATGTTAAAACGTATCAAAATTGTGACCAGC
R: gactgcagctactagTTAAAATGTTTCCCAGTTCTCCTCG
Source
The Tsr part was acquired by PCR from E. coli genomic DNA